Peroxiredoxin 2 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-peroxiredoxin-2-antibody-vpa00330.html?RenderPageSpecific=1
Peroxiredoxin 2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with peroxiredoxin 2 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to peroxiredoxin 2 (molecular weight 22 kDa)

Enlarge
  • Peroxiredoxin 2 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti Peroxiredoxin 2
  • Peroxiredoxin 2 Antibody with Control Lysate
  • Peroxiredoxin 2 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    Rabbit anti human peroxiredoxin 2 antibody recognizes the peroxiredoxin-2 protein, also known as epididymis secretory sperm binding protein Li 2a, natural killer cell-enhancing factor B, thiol-specific antioxidant 1, thioredoxin peroxidase 1 or torin. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00330WBdatasheet pdfdatasheet pdf100 µl
      VPA00330
      VPA00330KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00330K
      VPA00330KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00330KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human peroxiredoxin 2 antibody recognizes peroxiredoxin 2, also known as epididymis secretory sperm binding protein Li 2a, natural killer cell-enhancing factor B, thiol-specific antioxidant 1, thioredoxin peroxidase 1, thioredoxin-dependent peroxide reductase 1 and torin.

        Peroxiredoxin 2 is member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of the protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making Peroxiredoxin 2 a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13 (provided by RefSeq, Mar 2013).

        Rabbit anti Human peroxiredoxin 2 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti peroxiredoxin 2 detects a band of approximately 22 kDa in LNCaP cell lysate
        Anti peroxiredoxin 2 detects a band of approximately 22 kDa in LNCaP cell lysate
        Anti peroxiredoxin 2 detects a band of approximately 22 kDa in LNCaP cell lysate
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Ig fraction - liquid
        Ig fraction - liquid
        Ig fraction - liquid
      • Preparation
        Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 10 Westerns100μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 2 Westerns20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 10 Westerns100μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 2 Westerns20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH-conjugated synthetic peptide corresponding to aa 169-198 of human peroxiredoxin 2
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg LNCaP lysate lyophilized in RIPA buffer
        400μg LNCaP lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        hydrogen peroxide catabolic process
        oxidation-reduction process
        cell redox homeostasis
        removal of superoxide radicals
        thioredoxin peroxidase activity
        anti-apoptosis
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted