PEN2 Antibody

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PEN2 Antibody gallery image 1

Western Blot analysis of A-20 murine B-cell lymphoma cell line lysate probed with Rabbit anti Human PEN2 (AHP1197)

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PEN2 Antibody gallery image 2

Formalin fixed, paraffin embedded rat lung stained with Rabbit anti Human PEN2 (AHP1197) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • Rabbit anti Human PEN2 (N-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1197P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1197
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human PEN2 antibody recognises the amino terminal region of human Presenilin enhancer protein 2 (PEN), a ~12 kDa member of the PEN2 family and subunit of the gamma-secretase complex. This complex is an endoprotease that cleaves integral membrane proteins. These include Notch receptors and the beta-amyloid precursor protein which is cleaved to form amyloid beta peptide, a major component of the neuritic plaques found in Alzheimer’s disease. The gamma secretase complex consists of nicastrin, the presenilin homodimers and APH1. It is the addition of PEN2, the final stage of gamma secretase maturation that facilitates endoproteolysis of presenilin and confers proteolytic activity. PEN2 expression has been observed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.51.0ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1197 detects a band of approximately 12kDa in A-20 cell lysates.
    • Instructions For Use

    Additional PEN2 Antibody Formats

    Formats Applications Sizes available
    PEN2 Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Further Reading

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