PDIA3 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-pdia3-antibody-vpa00513.html?RenderPageSpecific=1
PDIA3 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PDIA3 antibody followed by detection with Donkey anti Sheep/Goat IgG (Fc):HRP (1/10,000, STAR88P) and visualized on the ChemiDoc™ MP with 119 second exposure. Arrow points to PDIA3 (molecular weight 58 kDa)

Enlarge
  • PDIA3 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti PDIA3
  • PDIA3 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    Goat anti-human PDIA3 antibody recognizes protein disulfide-isomerase A3, also known as 58 kDa glucose-regulated protein, 58 kDa microsomal protein, ER protein 57, ER protein 60, endoplasmic reticulum P58 or endoplasmic reticulum resident protein 57. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00513WBdatasheet pdfdatasheet pdf100 µl
      VPA00513
      VPA00513KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00513KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human PDIA3 antibody recognizes protein disulfide-isomerase A3, also known as 58 kDa glucose-regulated protein, 58 kDa microsomal protein, disulfide isomerase ER-60, epididymis secretory protein Li 269 or phospholipase C-alpha.

        The PDIA3 gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates (Trombetta and Helenius 1998) (provided by RefSeq, Jul 2008).

        Goat anti Human PDIA3 antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti PDIA3 detects a band of approximately 58 kDa in HepG2 cell lysates
        Anti PDIA3 detects a band of approximately 58 kDa in HepG2 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by affinity chromatography
        20μl Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide sequence C-TEYSALEQSTK
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        signal transduction
        protein disulfide isomerase activity
        protein import into nucleus
        protein retention in ER lumen
        protein binding
        post-translational protein modification
        endoplasmic reticulum lumen
        cysteine-type endopeptidase activity
        melanosome
        cell redox homeostasis
        protein N-linked glycosylation via asparagine
        phospholipase C activity
        protein folding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted
                • View more products with "PDIA3" specificity.