PDIA3 Antibody

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  • Goat anti PDIA3 (C-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1091E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1091
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human PDIA3 antibody recognises protein disulphide isomerase family A, member 3 (PDIA3), also known as 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57 or ERp57. PDIA3 is a ~55 kDa protein of the endoplasmic reticulum. PDIA3 interacts with lectin chaperones calreticulin and calnexin, modulating folding of newly synthesized glycoproteins by promoting the formation of disulphide bonds.

      PDIA3 expressed on the mouse sperm head is involved in sperm-egg fusion (Ellerman et al. 2006).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      Mouseyes
      RabbitExpected from Sequence
      HamsterExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human PDIA3 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence C-EEKPKKKKKAQEDL corresponding to the C-terminus of PDIA3 (NP_05304).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein import into nucleus
      signal transduction
      cell redox homeostasis
      protein binding
      protein N-linked glycosylation via asparagine
      melanosome
      protein retention in ER lumen
      protein disulfide isomerase activity
      post-translational protein modification
      cysteine-type endopeptidase activity
      endoplasmic reticulum lumen
      phospholipase C activity
      protein folding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/80000
      Western Blotting13ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1091 detects a band of approximately 55kDa in human Hepatoblastoma HepG2 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
    • Instructions For Use

    Additional PDIA3 Antibody Formats

    Formats Applications Sizes available
    PDIA3 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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