PDGF-B Antibody (PrecisionAbTM Antibody)

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PDGF-B Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PDGF-B antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 15 second exposure. Arrow points to PDGF-B (molecular weight 27 kDa)

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  • Rabbit anti PDGF-B
  • PDGF-B Antibody with Control Lysate
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    Rabbit anti-human PDGF-B antibody recognizes platelet-derived growth factor subunit B, also known as becaplermin, platelet-derived growth factor, beta polypeptide (oncogene SIS) or proto-oncogene c-Sis. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00534WBdatasheet pdfdatasheet pdf100 µl
      VPA00534
      VPA00534KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00534KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human PDGF-B antibody recognizes platelet-derived growth factor subunit B, also known as PDGF subunit B, becaplermin, platelet-derived growth factor 2, platelet-derived growth factor beta polypeptide or proto-oncogene c-Sis.

        The protein encoded by the PDGFB gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimer chains are connected by disulfide bonds.

        Mutations in PDGFB are associated with meningioma and Basal ganglia calcification, idiopathic, 5 (IBGC5), an autosomal dominant disorder characterized by progressive neurologic symptoms associated with brain calcifications (Keller et al. 2013). Reciprocal translocations between chromosomes 22 and 17, at sites where PDGFB and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans (DFSB) resulting from unregulated expression of growth factor (Kiuru-Kuhlefelt et al. 2001). Two alternatively spliced transcript variants encoding different isoforms have been identified for PDGFB (provided by RefSeq, Feb 2013).

        Rabbit anti Human PDGF-B antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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