PCBP2 Antibody (PrecisionAbTM Antibody)

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PCBP2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PCBP2 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 13 second exposure. Arrow points to PCBP2 (molecular weight 39 kDa)

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  • PCBP2 Antibody with Control Lysate
  • PCBP2 Antibody with Control Lysate
  • Rabbit anti PCBP2
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00661WBdatasheet pdfdatasheet pdf100 µl
    VPA00661
    VPA00661KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
    VPA00661K
    VPA00661KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00661KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Rabbit anti Human PCBP2 antibody recognizes the poly(rC)-binding protein 2, also known as alpha-CP2, heterogenous nuclear ribonucleoprotein (hnRNP) E2.

      The protein encoded by PCBP2 gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. PCBP2 and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for PCBP2 (provided by RefSeq, Jul 2008).

      Rabbit anti Human PCBP2 antibody detects a band of 39 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti PCBP2 detects a band of approximately 39 kDa in Jurkat cell lysates
      Rabbit anti PCBP2 detects a band of approximately 39 kDa in Jurkat cell lysates
      Rabbit anti PCBP2 detects a band of approximately 39 kDa in Jurkat cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
      Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
      Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
      Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
      Rabbit polyclonal antibody purified by affinity chromatography on Protein A
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide
      2% Sucrose
      0.09% Sodium Azide
      2% Sucrose
      0.09% Sodium Azide
      2% Sucrose
    • Immunogen
      Synthetic peptide encompassing part of the middle region of human PCBP2
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg Jurkat lysate lyophilized in RIPA buffer.
      400μg Jurkat lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      negative regulation of defense response to virus
      ribonucleoprotein complex
      response to virus
      nucleoplasm
      DNA binding
      negative regulation of type I interferon production
      cytosol
      RNA binding
      innate immune response
      ubiquitin protein ligase binding
      nuclear mRNA splicing, via spliceosome
      proteasomal ubiquitin-dependent protein catabolic process
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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