PAX2 Antibody | 3C7

PAX2 Antibody | 3C7 gallery image 1

Staining of paraffin-embedded human adrenal gland with Mouse anti Human PAX2 (MCA4953Z)

  • PAX2 Antibody | 3C7 thumbnail image 1
  • Mouse anti Human PAX2:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4953ZPdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human PAX2 antibody, clone 3C7 recognizes human Paired box protein Pax-2. Pax-2 is a 417 amino acid ~45 kDa nuclear protein critical to the development of the urinogential tract, eyes and CNS (Eccles et al. 2002).

      Mutations in the PAX2 gene can lead to deveolpment of Papillorenal syndrome (PAPRS) demonstrating both renal and ocular problems (Salomon et al. 2001, Higashide et al. 2005) or Focal segmental glomerulosclerosis 7 (FSGS7) showing segmental sclerosis of glomeruli (Barua et al. 2014).
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 194 - 304 of human PAX2.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      centriolar satellite
      metanephric mesenchymal cell differentiation
      mesenchymal to epithelial transition involved in metanephros morphogenesis
      promoter binding
      visual perception
      transcription activator activity
      transcription from RNA polymerase II promoter
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional PAX2 Antibody Formats

    Formats Clone Applications Sizes available
    PAX2 Antibody : Preservative Free 3C7 P 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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