PAX2 Antibody | 2E4

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PAX2 Antibody | 2E4 gallery image 1

Western blot analysis of PAX2 expression in PC-12 using Mouse anti Human PAX2:Azide Free (MCA3316Z)

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  • Mouse anti Human PAX2:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    2E4
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3316ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA3316Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human PAX2 antibody, clone 2E4 recognizes human Paired box protein Pax-2, also known as PAX2. PAX” is a 417 amino acid ~40 kDa transcription factor with a crucial role in the development of several organs including eyes, nervous system and urogenital tract and a possible role in the development of renal cells (UniProt : Q02962).

      Mutations in the PAX2 gene may lead to development of Papillorenal syndrome (PAPRS) a condition characterized by ocular and renal anomalies with multiple other possible presentations (Devriendt et al. 1998). Mutations in this gene may also lead to Focal segmental glomerulosclerosis 7 (FSGS7), a renal disease condition characterized by segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function which may progress to renal end-stage disease (Barua et al. 2014).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 194-304 of human PAX2
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      transcription from RNA polymerase II promoter
      nucleus
      mesenchymal to epithelial transition involved in metanephros morphogenesis
      visual perception
      promoter binding
      transcription activator activity
      centriolar satellite
      axonogenesis
      metanephric mesenchymal cell differentiation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional PAX2 Antibody Formats

    Formats Clone Applications Sizes available
    PAX2 Antibody : Preservative Free 2E4 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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