Parathyroid Hormone Related Peptide Antibody

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  • Rabbit anti Human Parathyroid Hormone Related Peptide
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2186E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human Parathyroid Hormone Related Peptide antibody specifically recognizes human Parathyroid hormone related peptide (PTHrP), a ubiquitously produced neuroendocrine polypeptide hormone, which signals through Parathyroid Hormone Receptor 1 (PTHR1), and acts as a critical regulator of cellular and tissue development, cell differentiation and migration, calcium and phosphorous extracellular concentrations, bone, teeth, and mammary gland development, and epithelial calcium ion transport.

      Studies have shown the involvement of PTHrP during the metastasis of colon cancer cells, identifying a positive correlation between PTHrP, Rac1 activity, the guanine nucleotide exchange factor Tiam1, and integrin alpha6beta4. Elevated expression of PTHrP is most commonly associated with breast cancer, and also with gastrointestinal (GI) tumours.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antiserum to human PTHrP was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant human PTHrP
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      negative regulation of chondrocyte differentiation
      cAMP metabolic process
      activation of adenylate cyclase activity by G-protein signaling pathway
      epidermis development
      positive regulation of cell proliferation
      positive regulation of cAMP biosynthetic process
      skeletal system development
      cell-cell signaling
      extracellular space
      negative regulation of cell proliferation
      hormone activity
      regulation of gene expression
      female pregnancy
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with AHP2186B as the detection reagent.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Parathyroid Hormone Related Peptide Antibody Formats

    Formats Applications Sizes available
    Parathyroid Hormone Related Peptide Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

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