PAI-1 Antibody (PrecisionAbTM Antibody)

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PAI-1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PAI-1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/50,000, STAR88P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to PAI-1 (molecular weight 43 kDa)

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  • PAI-1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti PAI-1
  • PAI-1 Antibody with Control Lysate
  • PAI-1 Antibody with Control Lysate
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    PAI-1 antibody recognizes plasminogen activator inhibitor 1, also known as PAI, PAI-1 or endothelial plasminogen activator inhibitor or Serpin E1. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00047WBdatasheet pdfdatasheet pdf100 µl
      VPA00047
      VPA00047KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00047K
      VPA00047KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00047KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human PAI-1 antibody recognizes human plasminogen activator inhibitor 1 (PAI-1), also known as Serpin E1. PAI-1 is a 402 amino acid serine protease inhibitor and the principle inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA)

        PAI-1 is present in plasma, platelets and endothelial cells, is expressed at increased levels in various disease states (Kruithof et al. 1988), and has been linked to an increased occurrence of thrombosis in obesity and coronary artery disease (Oseroff et al. 1989). Defects in PAI-1 are characterized by abnormal bleeding (Takahashi et al. 1996).

        Goat anti Human PAI-1 antibody recognizes PAI-1 as a single band of 43 kDa in a range of human cell lysates.
      • Target Species
        Human
      • Western Blotting
        Goat anti PAI-1 detects a band of approximately 43 kDa in MCF7 cell lysates.
        Goat anti PAI-1 detects a band of approximately 43 kDa in MCF7 cell lysates.
        Goat anti PAI-1 detects a band of approximately 43 kDa in MCF7 cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide with sequence C-GFKIDDKGMAPALRH, corresponding to the internal region of PAI-1.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg MCF7 lysate lyophilized in RIPA buffer.
        400μg MCF7 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        cellular response to chemical stimulus
        extracellular matrix
        negative regulation of smooth muscle cell migration
        positive regulation of interleukin-8 production
        negative regulation of vascular wound healing
        negative regulation of cell adhesion mediated by integrin
        platelet activation
        platelet degranulation
        extracellular space
        negative regulation of plasminogen activation
        chronological cell aging
        positive regulation of receptor-mediated endocytosis
        fibrinolysis
        serine-type endopeptidase activity
        regulation of receptor activity
        platelet alpha granule lumen
        protease binding
        negative regulation of fibrinolysis
        serine-type endopeptidase inhibitor activity
        negative regulation of apoptosis
        positive regulation of leukotriene production involved in inflammatory response
        negative regulation of smooth muscle cell-matrix adhesion
        defense response to Gram-negative bacterium
        positive regulation of monocyte chemotaxis
        positive regulation of angiogenesis
        cellular response to lipopolysaccharide
        plasma membrane
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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