PAI-1 Antibody

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PAI-1 Antibody gallery image 1

Western blot analysis of human liver lysate probed with Goat anti Human PAI-1 antibody (AHP1100)

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Immunohistochemical staining of formalin fixed, paraffin embedded human placenta stained with Goat anti Human PAI-1 antibody (AHP1100) following heat mediated antigen retrieval in citrate buffer (pH6.0)

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Immunohistochemical staining of formalin fixed, paraffin embedded human placenta stained with Goat anti Human PAI-1 antibody following heat mediated antigen retrieval in Tris/EDTA buffer (pH9.0)

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PAI-1 Antibody gallery image 4

ELISA analysis of human PAI-1 expression using Goat anti Human PAI-1 antibody (1.5μml-1 as a detection reagent in a sandwich ELISA configuration

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  • Goat anti Human PAI-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1100E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1100
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti human PAI-1 antibody recognizes human Plasminogen activator inhibitor 1 (PAI-1), also known as Serpin E1. PAI-1 is a 402 amino acid }45kDa serine protease inhibitor epitope within the internal region of human Serpin E1. PAI-1 (Serpin E1) is a 45kD serine protease inhibitor and the principle inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA)

      PAI-1 is present in plasma, platelets and endothelial cells, is expressed at increased levels in various disease states (Kruithof et al. 1988), and has been linked to an increased occurrence of thrombosis in obesity and coronary artery disease (Oseroff et al. 1989). Defects in PAI-1 are characterized by abnormal bleeding (Takahashi et al. 1996).

      Goat anti human PAI-1 antibody has been used successfully for the detection of PAI-1 in human tissues by immunohistochemistry, western blotting and ELISA.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/32000
      Western Blotting0.10.3 ug/ml
      Immunohistology - Paraffin(1)4.06.0 ug/ml
      (1)
      This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Citrate buffer pH6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human Placenta
    • Immunofluorescence
    • Western Blotting
      Goat anti human PAI-1 detects a band of approximately 45kDa in human duodenum, liver and lung lysates. A minimum incubation time of 1 hour is recommended with this antibody.
    • Instructions For Use

    Additional PAI-1 Antibody Formats

    Formats Applications Sizes available
    PAI-1 Antibody : Purified E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human Placenta

              References

              Further Reading

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