PAG Antibody (PrecisionAbTM Antibody)

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PAG Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with PAG antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 141 second exposure. Arrow points to PAG (molecular weight 80 kDa)

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  • PAG Antibody with Control Lysate
  • Mouse anti PAG
  • PAG Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    PAG antibody recognizes phosphoprotein associated with glycosphingolipid-enriched microdomains 1, also known as Csk-binding protein, transmembrane adapter protein, PAG, or transmembrane phosphoprotein Cbp. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00028WBdatasheet pdfdatasheet pdf100 µl
      VMA00028
      VMA00028KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00028K
      VMA00028KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00028KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human PAG antibody recognizes human phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), also known as Csk-binding protein (Cbp) or transmembrane phosphoprotein Cbp. PAG is a single pass type III transmembrane protein. Mouse anti Human PAG antibody recognizes an epitope located in the cytoplasmic domain which localizes to membrane lipid rafts and is involved in regulation of T-Cell activation.

        The engagement of immunoreceptors with natural ligands or antibodies is the initiating factor in a long line of events down a cytosolic signaling cascade. The transmission of signals along the various pathways within this cascade requires the phosphorylation of various substrates, by members of the src and syk families of protein tyrosine kinases (PTKs).

        Evidence has shown that by binding to the SH2 domain of the protein tyrosine kinase Csk, a major negative regulator of src-family kinases, PAG acts not only acts as a ‘co-factor’ for relocating Csk to substrate rich microdomains, but also increases the affinity of Csk for src-family kinases, through the formation of PAG-Csk complexes.

        Mouse anti Human PAG antibody recognizes human PAG as a single band of ~80 kDa in human cell line lysates by Western blotting under reducing conditions.
      • Target Species
        Human
      • Western Blotting
        Anti PAG antibody detects a band of approximately 80 kDa in Raji cell lysates.
        Anti PAG antibody detects a band of approximately 80 kDa in Raji cell lysates.
        Anti PAG antibody detects a band of approximately 80 kDa in Raji cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
        Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant protein corresponding to amino acids 97 - 432 of human Cbp/PAG
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Raji lysate lyophilized in RIPA buffer.
        400μg Raji lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        T cell receptor signaling pathway
        plasma membrane
        SH3/SH2 adaptor activity
        epidermal growth factor receptor signaling pathway
        membrane raft
        intracellular signal transduction
        SH2 domain binding
        integral to membrane
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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