PAG Antibody | MEM-255

PAG Antibody | MEM-255 gallery image 1

Published Investigator image:
Mouse anti Human PAG antibody, clone MEM-255 used to demonstrate knockdown of PAG protein in PAGsi cell lystates by western blotting
Image caption:
CTLA-4 suppression restores the defect in proliferation caused by PAG suppression. Primary human T cells were transfected with siRNAs targeting Renilla (ctrl), PAG (PAGsi), CTLA-4 (CTLA4si) or PAG + CTLA-4 (PAGsi+CTLA4si). Cells were then stimulated with indicated stimuli for three days on a plastic plate. (A) To demonstrate the downregulation of protein expression by siRNAs, total cell lysate staining for PAG and CTLA-4 is shown.

From: Smida M, Cammann C, Gurbiel S, Kerstin N, Lingel H, Lindquist S, Simeoni L, Brunner-Weinzierl MC, Suchanek M, Schraven B, Lindquist JA.
PAG/Cbp suppression reveals a contribution of CTLA-4 to setting the activation threshold in T cells.
Cell Commun Signal. 2013 Apr 19;11(1):28.

  • PAG Antibody | MEM-255 thumbnail image 1
  • Mouse anti Human PAG
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2422WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human PAG antibody, clone MEM-255 recognizes humanr Phosphoprotein Associated with Glycosphingolipid-enriched microdomains (PAG), also known as csk-binding protein (Cbp) or Transmembrane phosphoprotein Cbp. PAG is a single pass type II transmembrane protein. Mouse anti Human PAG antibody, clone MEM-255 recognizes an epitope (aa 235-280) located in the cytoplasmic domain which localizes to membrane lipid rafts and is involved in regulation of T-Cell activation.

      The engagement of immunoreceptors with natural ligands or antibodies is the initiating factor in a long line of events down a cytosolic signaling cascade. The transmission of signals along the various pathways within this cascade requires the phosphorylation of various substrates, by members of the src and syk families of protein tyrosine kinases (PTKs).

      Evidence has shown that by binding to the SH2 domain of the PTK csk, a major negative regulator of src-family kinases, PAG acts not only acts as a ‘co-factor’ for relocating csk to substrate rich microdomains, but also increases the affinity of csk for src-family kinases, through the formation of PAG-csk complexes.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Recombinant intracellular fragment (amino acids 97-432) of human Cbp/PAG
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      SH2 domain binding
      epidermal growth factor receptor signaling pathway
      integral to membrane
      SH3/SH2 adaptor activity
      intracellular signal transduction
      T cell receptor signaling pathway
      membrane raft
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2422 detects a band of approximately 50 kDa in Ramos cell lysates.
    • Instructions For Use

    Additional PAG Antibody Formats

    Formats Clone Applications Sizes available
    PAG Antibody : Purified MEM-255 WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting


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