p70S6K Antibody

p70S6K Antibody gallery image 1

Western blotting analysis of human placenta lysate (RIPA buffer, 35μg total protein per lane) probed with Goat anti Human p70S6K antibody (AHP1575) used at 0.05μgml-1. Primary incubated for 1 hour. Signal was detected by western blot using chemiluminescence

p70S6K Antibody gallery image 2

Western blotting analysis of rat brain lysate (35μg protein in RIPA buffer) probed with Goat anti Human p70S6K antibody (AHP1575) used at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

p70S6K Antibody gallery image 3

Western blotting analysis of NIH3T3 cell lysate (35μg protein in RIPA buffer) probed with Goat anti p70S6K antibody (AHP1575) used at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

p70S6K Antibody gallery image 4

Immunohistochemical staining of paraffin embedded human kidney following heat mediated antigen retrieval with citrate buffer at pH 6.0 using Goat anti p70S6K (AHP1575) at 2.0μgml-1, followed by anti Goat - HRP staining and DAB chromogen development

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  • Goat anti Human p70S6K (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1575E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti phospho-70S6K antibody recognizes an epitope within the C-terminal (CT) region of human p70S6K, otherwise known as RPS6KB1 or Ribosomal protein S6 kinase beta-1. 70S6K is a serine/threonine kinase which acts upon 40S ribosomal protein S6 to induce protein synthesis, allowing for efficient G1 phase progression.

      p70S6K is activated following phosphorylation of several sites, in response to hormones, growth factors, mitogenic stimuli and physical exercise, and is a downstream effector in the AKT/mTOR (mammalian target of rapamycin) signalling pathway. p70S6K also acts as a regulator of cell motility, invasion, and cytoskeletal reorganization.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.21.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1575 detects a band of approximately 65-70kDa in human muscle and placenta cell lysates.
    • Instructions For Use

    Additional p70S6K Antibody Formats

    Formats Applications Sizes available
    p70S6K Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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