p53DINP1 Antibody

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p53DINP1 Antibody gallery image 1

Western blot analysis of lysate from human lung probed with Rabbit anti p53DINP1 (AHP1659)

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p53DINP1 Antibody gallery image 2

Immunohistochemical staining of mouse liver with Rabbit anti p53DINP1 (AHP1659)

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  • Rabbit anti p53DINP1
  • Rabbit anti p53DINP1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1659P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1659
    AHP1659TP *, WBdatasheet pdfdatasheet pdf50 µg
    AHP1659T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti p53DINP1 antibody recognizes p53DINP1 (p53-dependent damage-inducible nuclear protein 1). The protein promotes p53 phosphorylation on Ser46 in response to DNA double strand breaks, thereby inducing apoptosis. p53DINP1 is found in the nucleus and is ubiquitously expressed. Expression is dramatically reduced in pancreatic ductal adenocarcinomas early in the development of pancreatic cancer.

      Two isoforms of the protein exists, a 27kDa alpha and an 18kDa beta form. AHP1659 detects a band of 27kDa in human lung tissue lysate. In human spleen, and mouse liver and kidney tissue a band is detected at 18kDa.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to p53DINP1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A peptide corresponding to 14 amino acids near the amino terminus of human p53DINP1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.02.0ug/ml
      Immunohistology - Paraffin(1)2ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.02.0ug/ml
      Immunohistology - Paraffin(1)2ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional p53DINP1 Antibody Formats

    Formats Applications Sizes available
    p53DINP1 Antibody : Purified P *, WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      STAR36D680GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

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