p27/Kip1 Antibody (PrecisionAbTM Antibody)

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p27/Kip1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with p27/kip1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to p27/kip1 (molecular weight 27 kDa)

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  • p27/Kip1 Antibody with Control Lysate
  • Rabbit anti p27/Kip1
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00721WBdatasheet pdfdatasheet pdf100 µl
    VPA00721
    VPA00721KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00721KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Rabbit anti Human p27/kip1 antibody recognizes the cyclin-dependent kinase inhibitor 1, also known as CDK-interacting protein 1, KIP1, BWS, P27KIP1 or wild-type p53-activated fragment 1.

      The CDKN1A gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of CDKN1A is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for CDKN1A (provided by RefSeq, Nov 2010).

      Rabbit anti Human p27/kip1 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti p27/kip1 detects a band of approximately 27 kDa in NIH/3T3 cell lysates
      Rabbit anti p27/kip1 detects a band of approximately 27 kDa in NIH/3T3 cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Rabbit polyclonal antibody purified by affinity chromatography
      Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      TRIS buffered glycine
      TRIS buffered glycine
    • Preservative Stabilisers
      0.01% Thiomersal
      <50% Glycerol
      0.01% Thiomersal
      <50% Glycerol
    • Immunogen
      Recombinant protein encompassing part of the center region of human p27/kip1
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg NIH/3T3 lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      cytosol
      G1 phase of mitotic cell cycle
      S phase of mitotic cell cycle
      cellular response to lithium ion
      DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
      negative regulation of cell proliferation
      phosphatidylinositol-mediated signaling
      negative regulation of cell growth
      cyclin-dependent protein kinase inhibitor activity
      regulation of cyclin-dependent protein kinase activity
      G1/S transition of mitotic cell cycle
      nucleoplasm
      cell cycle arrest
      autophagic cell death
      transforming growth factor beta receptor, cytoplasmic mediator activity
      nerve growth factor receptor signaling pathway
      protein phosphatase binding
      negative regulation of gene-specific transcription
      induction of apoptosis
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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