p27/Kip1 Antibody (PrecisionAbTM Antibody)

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p27/Kip1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with p27/Kip1 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 30 second exposure. Arrow points to p27/Kip1 (molecular weight 27 kDa).

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  • p27/Kip1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti p27/Kip1
  • p27/Kip1 Antibody with Control Lysate
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    p27/Kip1 antibody recognizes cyclin-dependent kinase inhibitor 1, also known as CDK-interacting protein 1, KIP1, BWS, P27KIP1 or wild-type p53-activated fragment 1.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00657WBdatasheet pdfdatasheet pdf100 µl
      VMA00657
      VMA00657KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00657KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human p27/Kip1 antibody recognizes cyclin-dependent kinase inhibitor 1, also known as CDK-interacting protein 1, KIP1, BWS, P27KIP1 or wild-type p53-activated fragment 1.

        CDKN1A encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of CDKN1A is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for CDKN1A (provided by RefSeq, Nov 2010).

        Mouse anti Human p27/Kip1 antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti p27/Kip1 antibody detects a band of approximately 27 kDa in C6 cell lysates
        Mouse anti p27/Kip1 antibody detects a band of approximately 27 kDa in C6 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
        20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Full length recombinant human p27/Kip1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg C6 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        negative regulation of cell growth
        cytosol
        G1/S transition of mitotic cell cycle
        cyclin-dependent protein kinase inhibitor activity
        phosphatidylinositol-mediated signaling
        nerve growth factor receptor signaling pathway
        autophagic cell death
        transforming growth factor beta receptor, cytoplasmic mediator activity
        negative regulation of gene-specific transcription
        negative regulation of cell proliferation
        nucleoplasm
        protein phosphatase binding
        G1 phase of mitotic cell cycle
        S phase of mitotic cell cycle
        cell cycle arrest
        DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
        induction of apoptosis
        cellular response to lithium ion
        regulation of cyclin-dependent protein kinase activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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