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p27/Kip1 Antibody

p27/Kip1 Antibody gallery image 1

Western blot analysis of (A) A431, (B) HL60 and (C) HeLa whole cell lysates probed with Rabbit anti p27/Kip1

  • p27/Kip1 Antibody thumbnail image 1
  • Rabbit anti p27/Kip1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2409WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti p27/Kip1 antibody recognizes cyclin-dependent kinase inhibitor p27 (p27), also known as cyclin-dependent kinase inhibitor 1B or p27Kip1. The protein is a cyclin-dependent kinase inhibitor (CKI) that belongs to the Cip/Kip protein family (Cerqueira et al. 2014). As the name implies, CKIs play a role in the regulation of cyclin-dependent kinases (CDKs) and cyclin-CDK complexes (Cerqueira et al. 2014).

      Aberrant p27Kip1. levels have been reported in breast and colorectal cancers (Sgambato et al. 1997, Ogino et al. 2007).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti p27/Kip1 reconizes a band of approximately 27 kDa when run under reducing conditions
    • Instructions For Use

    Additional p27/Kip1 Antibody Formats

    Formats Applications Sizes available
    p27/Kip1 Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
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      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • p27/Kip1 Antibody thumbnail image 1

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