Osteocalcin antibody | 2H9F11F8
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Mouse anti Human Osteocalcin
- Product Type
- Monoclonal Antibody
- Clone
- 2H9F11F8
- Isotype
- IgG2a
- Specificity
- Osteocalcin
Antibody Quality and Performance Guarantee
| Mouse anti Human Osteocalcin antibody, clone 2H9F11F8 recognizes intact human and bovine osteocalcin in a "2 site" assay and reacts with osteocalcin fragments corresponding to amino acids 15-31 and 20-43 of the native molecule. It does not recognize fragments representing amino acids 1-19 or 7-19.Osteocalcin (Bone Gla Protein) is a 49 amino acid single chain vitamin K dependent protein (MW 5.8 kDa), made by osteoblasts that is a major component of the non-collagenous bone matrix. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget’s disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Pig Mouse Rat Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Recombinant human osteocalcin GST fusion protein.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Osteocalcin antibody
-
Rehder, D.S. et al. (2015) Gamma-carboxylation and fragmentation of osteocalcin in human serum defined by mass spectrometry.
Mol Cell Proteomics. 14 (6): 1546-55. -
Käkönen, S-M. et al. (2005) Method for prediction of bone fractures by osteocalcin measurements
US Patent: US6967081B1
- Synonyms
- Bone GLA Protein
- RRID
- AB_617357
- UniProt
- P02818
- Entrez Gene
- BGLAP
- GO Terms
- GO:0005509 calcium ion binding
- GO:0007155 cell adhesion
- GO:0008147 structural constituent of bone
- GO:0030282 bone mineralization
- GO:0030500 regulation of bone mineralization
- GO:0042476 odontogenesis
- GO:0045124 regulation of bone resorption
- GO:0045670 regulation of osteoclast differentiation
- GO:0046848 hydroxyapatite binding
- View More GO Terms
0400-0041
163604
1708
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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