Osteocalcin Antibody | 2D4

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Osteocalcin Antibody | 2D4 gallery image 1

Immunofluorescence image showing osteocalcin expression in HeLa cells using Mouse anti Human osteocalcin antibody, clone 2D4 (MCA5225Z)

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  • Osteocalcin Antibody | 2D4 thumbnail image 1
  • Mouse anti Human Osteocalcin:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    2D4
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5225ZIFdatasheet pdfdatasheet pdf0.1 mg
    MCA5225Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human osteocalcin antibody, clone 2D4 recognizes human osteocalcin, also known as bone gla protein or Gamma-carboxyglutamic acid-containing protein. Osteocalcin is a 49 amino acid ~5 kDa calcium-binding bone protein comprising ~1-2% total bone protein mass. Osteocalcin also has an additional 28 amino acid propeptide region along with a 23 amino acid signal peptide region.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 52-100 of human osteocalcin.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      regulation of bone resorption
      regulation of osteoclast differentiation
      bone mineralization
      hydroxyapatite binding
      structural constituent of bone
      regulation of bone mineralization
      odontogenesis
      calcium ion binding
      cell adhesion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Osteocalcin Antibody Formats

    Formats Clone Applications Sizes available
    Osteocalcin Antibody : Preservative Free 2D4 IF 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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