ORC4L Antibody

ORC4L Antibody gallery image 1

Western blot analysis of ORC4L in Jurkat cell lysate probed with Goat anti ORC4L antibody (AHP638)

ORC4L Antibody gallery image 2

Western blotting analysis of Jurkat Nuclear lysate (35μg protein in RIPA buffer) probed with Goat anti Human ORC4L antibody (AHP638) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

ORC4L Antibody gallery image 3

Western blotting of mouse heart lysate (35μg protein in RIPA buffer) probed with Goat anti Human ORC4L antibody (AHP638) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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  • Goat anti ORC4L (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP638WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti ORC4L antibody recognises origin recognition complex subunit 4-like (ORC4L) protein. ORC4L is required for initiation of DNA replication with a putative nucleotide triphosphate binding motif.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to ORC4L were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence DVRQWATSSLSWL corresponding to the C-terminus of ORC4L.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      nucleoside-triphosphatase activity
      cell cycle checkpoint
      DNA replication origin binding
      G1/S transition of mitotic cell cycle
      nuclear origin of replication recognition complex
      M/G1 transition of mitotic cell cycle
      S phase of mitotic cell cycle
      DNA-dependent DNA replication initiation
      protein binding
      ATP binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1ug/ml3ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP638 detects a band of approximately 40-45kDa in Jurkat and NSO (mouse) cell lysates.
    • Instructions For Use

    Additional ORC4L Antibody Formats

    Formats Applications Sizes available
    ORC4L Antibody : Purified WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting


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