Western blotting analysis of Jurkat Nuclear lysate (35μg protein in RIPA buffer) probed with Goat anti Human ORC4L antibody (AHP638) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Western blotting of mouse heart lysate (35μg protein in RIPA buffer) probed with Goat anti Human ORC4L antibody (AHP638) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Goat anti ORC4L antibody recognises origin recognition complex subunit 4-like (ORC4L) protein. ORC4L is required for initiation of DNA replication with a putative nucleotide triphosphate binding motif.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antisera to ORC4L were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Bovine Serum Albumin
Peptide sequence DVRQWATSSLSWL corresponding to the C-terminus of ORC4L.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP638 detects a band of approximately 40-45kDa in Jurkat and NSO (mouse) cell lysates.