Oncostatin M Antibody

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Formalin fixed, paraffin embedded human breast malignant adenocarcinoma stained with Rabbit anti Human oncostatin M (AHP791 following enzyme mediated antigen retrieval using proteinase K

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Oncostatin M Antibody gallery image 2

Formalin fixed, paraffin embedded human breast malignant adenocarcinoma stained with Rabbit anti Human oncostatin M (AHP791 following enzyme mediated antigen retrieval using proteinase K

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  • Rabbit anti Human Oncostatin M:Biotin
  • Rabbit anti Human Oncostatin M
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP791E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP791
    AHP791BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP791B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human Oncostatin M antibody recognizes human Oncostatin M (OSM), a 209 amino acid pleiotropic cytokine, originally isolated from PMA (phorbol 12-myristate 13-acetate) treated U937 leukaemia cells and mitogen-activated T lymphocytes.

      OSM was identified by the ability to inhibit the replication of various human tumour cell lines, but has also been shown to exert a stimulatory effect on fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, and to induce the release of certain cytokines by endothelial cells. OSM signals through binding with low affinity to the gp130 protein present in two cell surface receptors: type I consisting of gp130 and LIFR (leucocyte inhibitory factor receptor) which also acts as a receptor for LIF, and type II consisting of gp130 and OSMR, which mediates OSM specific activities.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
    • Preparation
    • Antiserum Preparation
      Antisera to human Oncostatin M were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
      Recombinant human Oncostatin M.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 50ug/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of MAPKKK cascade
      extracellular space
      positive regulation of cell proliferation
      oncostatin-M receptor binding
      cell proliferation
      negative regulation of cell proliferation
      cytokine activity
      positive regulation of peptidyl-tyrosine phosphorylation
      positive regulation of acute inflammatory response
      immune response
      oncostatin-M receptor complex
      positive regulation of peptidyl-serine phosphorylation
      regulation of growth
      negative regulation of hormone secretion
      positive regulation of transcription from RNA polymerase II promoter
      growth factor activity
      positive regulation of cell division
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.150.30ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml
      Functional Assays0.100.175ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Oncostatin M Antibody Formats

    Formats Applications Sizes available
    Oncostatin M Antibody : Purified E, FN, P *, WB 0.1 mg
    Oncostatin M Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Further Reading

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