NUP62 Antibody (PrecisionAbTM Antibody)

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NUP62 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with NUP62 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to NUP62 (molecular weight 72 kDa)

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  • NUP62 Antibody with Control Lysate
  • NUP62 Antibody with Control Lysate
  • Rabbit anti NUP62
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00728WBdatasheet pdfdatasheet pdf100 µl
    VPA00728
    VPA00728KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
    VPA00728K
    VPA00728KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00728KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Rabbit anti Human NUP62 antibody recognizes the nuclear pore glycoprotein p62, also known as 62 kDa nucleoporin or nucleoporin Nup62.

      The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by NUP62 gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of NUP62 encode a single protein isoform (provided by RefSeq, Jul 2008).

      Rabbit anti Human NUP62 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti NUP62 detects a band of approximately 72 kDa in MOLT-4 cell lysates
      Rabbit anti NUP62 detects a band of approximately 72 kDa in MOLT-4 cell lysates
      Rabbit anti NUP62 detects a band of approximately 72 kDa in MOLT-4 cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
      Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
      Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
      Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
      Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      TRIS buffered glycine
      TRIS buffered glycine
      TRIS buffered glycine
    • Preservative Stabilisers
      0.01% Thiomersal
      <50% Glycerol
      0.01% Thiomersal
      <50% Glycerol
      0.01% Thiomersal
      <50% Glycerol
    • Immunogen
      Recombinant protein encompassing part of the middle region of human NUP62
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg MOLT-4 lysate lyophilized in RIPA buffer.
      400μg MOLT-4 lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      cytoplasm
      transcription regulator activity
      negative regulation of cell proliferation
      ubiquitin binding
      nuclear pore
      transmembrane transport
      carbohydrate metabolic process
      mRNA transport
      protein serine/threonine kinase activity
      nucleocytoplasmic shuttling complex
      structural constituent of nuclear pore
      receptor signaling complex scaffold activity
      viral reproduction
      cell surface receptor linked signaling pathway
      nuclear membrane
      cell death
      spindle pole
      positive regulation of epidermal growth factor receptor signaling pathway
      hormone-mediated signaling pathway
      ribonucleoprotein complex
      glucose transport
      protein transport
      SH2 domain binding
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      positive regulation of transcription
      thyroid hormone receptor binding
      chromatin binding
      negative regulation of apoptosis
      regulation of glucose transport
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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