Nucleoside Diphosphate Kinase A Antibody (PrecisionAbTM Antibody)

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Nucleoside Diphosphate Kinase A Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with nucleoside diphosphate kinase A antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 2.67 second exposure. Arrow points to nucleoside diphosphate kinase A (molecular weight 17 kDa)

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  • Nucleoside Diphosphate Kinase A Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Nucleoside Diphosphate Kinase A Antibody with Control Lysate
  • Nucleoside Diphosphate Kinase A Antibody with Control Lysate
  • Mouse anti Nucleoside Diphosphate Kinase A
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    Nucleoside diphosphate Kinase A antibody recognizes nucleoside diphosphate kinase A, also known as NDP kinase A, granzyme A-activated DNase, metastasis inhibition factor nm23 or tumor metastatic process-associated protein. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2b
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00345WBdatasheet pdfdatasheet pdf100 µl
      VMA00345
      VMA00345KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00345K
      VMA00345KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00345KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human nucleoside diphosphate kinase A antibody recognizes nucleoside diphosphate kinase A, also known as NDP kinase A, granzyme A-activated DNase, metastasis inhibition factor nm23, non-metastatic cells 1 and tumor metastatic process-associated protein.

        The NME1 gene was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by NME2) isoforms. Mutations in NME1 have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for NME1. Co-transcription of NME1 and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product (provided by RefSeq, Jul 2008).

        Mouse anti Human nucleoside diphosphate kinase A antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti nucleoside diphosphate kinase A detects a band of approximately 17 kDa in HeLa cell lysates
        Anti nucleoside diphosphate kinase A detects a band of approximately 17 kDa in HeLa cell lysates
        Anti nucleoside diphosphate kinase A detects a band of approximately 17 kDa in HeLa cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Mouse monoclonal antibody prepared by affinity chromatography on Protein G
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant human nucleoside diphosphate kinase A
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        GTP binding
        cell differentiation
        magnesium ion binding
        cytosol
        regulation of apoptosis
        nucleobase, nucleoside and nucleotide interconversion
        CTP biosynthetic process
        endocytosis
        GTP biosynthetic process
        nucleoside diphosphate kinase activity
        deoxyribonuclease activity
        nucleus
        UTP biosynthetic process
        negative regulation of cell proliferation
        ATP binding
        positive regulation of epithelial cell proliferation
        identical protein binding
        positive regulation of DNA binding
        nervous system development
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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