NR1D1 Antibody | 4F6

NR1D1 Antibody | 4F6 gallery image 1

Staining of paraffin-embedded human kidney with Mouse anti Human NR1D1 (MCA5045Z)

NR1D1 Antibody | 4F6 gallery image 2

Immunofluorescence showing NR1D1in HeLa cells using Mouse anti Human NR1D1 (MCA5045Z)

NR1D1 Antibody | 4F6 gallery image 3

Western blot analysis of NR1D1 expression in an over-expressed 293 cell lysate either non-ransfected (lane 1) or transfected with chimera RNAi (lane 2) demonstrating knockdown of NR1D1 expression using Mouse anti Human NR1D1 antibody, clone 4F6 (MCA5045Z). GAPDH expression is included as a specificity and loading control.

  • NR1D1 Antibody | 4F6 thumbnail image 1
  • NR1D1 Antibody | 4F6 thumbnail image 2
  • NR1D1 Antibody | 4F6 thumbnail image 3
  • Mouse anti Human NR1D1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5045ZIF, P, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human NR1D1 antibody, clone 4F6 recognizes human Nuclear receptor subfamily 1 group D member 1 (NR1D1), also known as Rev-erbA-alpha, V-erbA-related protein 1 or EAR-1. NR1D1 is a 614 amino acid ~68 kDa transcriptional repressor involved in the regulation of circadian rythm and metabolic processes in a heme dependent manner (UniProt: P20393).

      NR1D1 is widely expressed, at high levels in adipose tissue, liver, skeletal muscle and brain. Defects in NR1D1 mat be associated with cancer and it appears to fulfill a prosurvival role in some breast cancers (Kourtidis et al. 2010).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to full length human NR1D1 with a 26 kDa GST tag.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      negative regulation of receptor biosynthetic process
      promoter binding
      regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
      steroid hormone receptor activity
      negative regulation of gene-specific transcription from RNA polymerase II promoter
      transcription corepressor activity
      nuclear chromatin
      zinc ion binding
      cellular response to lipopolysaccharide
      negative regulation of toll-like receptor 4 signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Immunohistology - Paraffin0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NR1D1 Antibody Formats

    Formats Clone Applications Sizes available
    NR1D1 Antibody : Preservative Free 4F6 IF, P, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


              Write your review

              You may also be interested in...

              You May Also Be Interested In...

              You may also like You may also like

              CD220 Antibodies1 products