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NR1D1 Antibody | 4F6

NR1D1 Antibody | 4F6 gallery image 1

Staining of paraffin-embedded human kidney with Mouse anti Human NR1D1 (MCA5045Z)

NR1D1 Antibody | 4F6 gallery image 2

Immunofluorescence showing NR1D1in HeLa cells using Mouse anti Human NR1D1 (MCA5045Z)

NR1D1 Antibody | 4F6 gallery image 3

Western blot analysis of NR1D1 expression in an over-expressed 293 cell lysate either non-ransfected (lane 1) or transfected with chimera RNAi (lane 2) demonstrating knockdown of NR1D1 expression using Mouse anti Human NR1D1 antibody, clone 4F6 (MCA5045Z). GAPDH expression is included as a specificity and loading control.

  • NR1D1 Antibody | 4F6 thumbnail image 1
  • NR1D1 Antibody | 4F6 thumbnail image 2
  • NR1D1 Antibody | 4F6 thumbnail image 3
  • Mouse anti Human NR1D1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5045ZIF, P, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human NR1D1 antibody, clone 4F6 recognizes human Nuclear receptor subfamily 1 group D member 1 (NR1D1), also known as Rev-erbA-alpha, V-erbA-related protein 1 or EAR-1. NR1D1 is a 614 amino acid ~68 kDa transcriptional repressor involved in the regulation of circadian rythm and metabolic processes in a heme dependent manner (UniProt: P20393).

      NR1D1 is widely expressed, at high levels in adipose tissue, liver, skeletal muscle and brain. Defects in NR1D1 mat be associated with cancer and it appears to fulfill a prosurvival role in some breast cancers (Kourtidis et al. 2010).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to full length human NR1D1 with a 26 kDa GST tag.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      negative regulation of receptor biosynthetic process
      promoter binding
      regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
      steroid hormone receptor activity
      negative regulation of gene-specific transcription from RNA polymerase II promoter
      transcription corepressor activity
      nuclear chromatin
      zinc ion binding
      cellular response to lipopolysaccharide
      negative regulation of toll-like receptor 4 signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Immunohistology - Paraffin0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NR1D1 Antibody Formats

    Formats Clone Applications Sizes available
    NR1D1 Antibody : Preservative Free 4F6 IF, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • NR1D1 Antibody | 4F6 thumbnail image 1
                • NR1D1 Antibody | 4F6 thumbnail image 2
                • NR1D1 Antibody | 4F6 thumbnail image 3


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