Western blotting analysis of human kidney lysate (35µg total protein in RIPA buffer) probed with Goat anti Human NQO1 antibody (AHP2022) at 0.03μgml-1. Primary incubated for 1 hour. Signal was detected by chemiluminescence
Western blotting analysis of rat (A) and pig (B) kidney lysate (35μg protein in RIPA buffer) probed with Goat anti Human NQO1 (AHP2022) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Goat anti Human NQO1 antibody recognizes human NAD(P)H dehydrogenase [quinone] 1, also known as NQO1, Azoreductase, DT-diaphorase DTD, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase or Quinone reductase 1. NQO1 is a 273 amino acid ~30 kDa cytoplasmic enzyme involved in quinone reduction.
Multiple isoforms of NQO1 generated by alternative splicing have been described. Goat anti Human NQO1 antibody (AHP2022) is expected to recognize all described isoforms (UniProt : P15559)
NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumours including lung, brain, liver, colon and breast (Bey et al. 2007). The ability of NQO1 to bioactivate anti-tumour quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies (Kelsey et al. 1997). Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity (Smith et al. 2001).
Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antiserum to human NQO1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Synthetic peptide sequence C-SIPTDNQIKARK from the C–terminal region of NQO1 (NP_000894.1; NP_001020604.1; NP_001020605.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP2022 detects a band of approximately 30kDa in human kidney and lung cell lysates.