NQO1 Antibody

NQO1 Antibody gallery image 1

Western blotting analysis of human kidney lysate (35µg total protein in RIPA buffer) probed with Goat anti Human NQO1 antibody (AHP2022) at 0.03μgml-1. Primary incubated for 1 hour. Signal was detected by chemiluminescence

NQO1 Antibody gallery image 2

Western blotting analysis of rat (A) and pig (B) kidney lysate (35μg protein in RIPA buffer) probed with Goat anti Human NQO1 (AHP2022) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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  • Goat anti Human NQO1 (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2022E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human NQO1 antibody recognizes human NAD(P)H dehydrogenase [quinone] 1, also known as NQO1, Azoreductase, DT-diaphorase DTD, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase or Quinone reductase 1. NQO1 is a 273 amino acid ~30 kDa cytoplasmic enzyme involved in quinone reduction.

      Multiple isoforms of NQO1 generated by alternative splicing have been described. Goat anti Human NQO1 antibody (AHP2022) is expected to recognize all described isoforms (UniProt : P15559)

      NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumours including lung, brain, liver, colon and breast (Bey et al. 2007). The ability of NQO1 to bioactivate anti-tumour quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies (Kelsey et al. 1997). Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity (Smith et al. 2001).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human NQO1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-SIPTDNQIKARK from the C–terminal region of NQO1 (NP_000894.1; NP_001020604.1; NP_001020605.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      NAD(P)H dehydrogenase (quinone) activity
      nitric oxide biosynthetic process
      response to toxin
      electron carrier activity
      synaptic transmission, cholinergic
      regulation of cellular amino acid metabolic process
      oxidation-reduction process
      xenobiotic metabolic process
      coenzyme binding
      cytochrome-b5 reductase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.030.1ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP2022 detects a band of approximately 30kDa in human kidney and lung cell lysates.
    • Instructions For Use

    Additional NQO1 Antibody Formats

    Formats Applications Sizes available
    NQO1 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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