NQO1 Antibody | A180

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Hela lysate probed with Mouse anti Human NQO1 (MCA2880)

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Hela lysate probed with Mouse anti Human NQO1:HRP (MCA2880P)

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  • Product Type
    Monoclonal Antibody
  • Clone
    A180
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2880PP *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2880P
    MCA2880GAIP, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2880GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti human NQO1 antibody, clone A180 specifically recognises human NQO1 (NAD(P)H:quinone oxidoreductase 1), a 31kDa cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones.

      NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumours including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumour quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies. Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele (Nerbert, D.W. et al. 2002). Clone A180 recognizes the product of the wild-type allele NQO1*1 but does not recognize the product of the mutant NQO1*2 allele (Sato, M. et al. 2010 and Fegerholm, R. et al. 2008).

      Mouse anti human NQO1 antibody, clone A180 detects endogenous levels of total NQO1, and does not cross-react with NQO2 (Siegal, D. et al. 1998).

      Clone A180 is reported as suitable for use in immunofluorescence studies.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseno
      RatWeak
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.01% Thiomersal
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Purified recombinant human NQO1
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      regulation of cellular amino acid metabolic process
      synaptic transmission, cholinergic
      cytosol
      oxidation-reduction process
      electron carrier activity
      cytochrome-b5 reductase activity
      nitric oxide biosynthetic process
      coenzyme binding
      NAD(P)H dehydrogenase (quinone) activity
      response to toxin
      xenobiotic metabolic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1001/250
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting1/1001/1000
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human lung
    • Histology Positive Control Tissue
      Human lung
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      MCA2880P detects a band of approximately 31kDa in HeLa and HepG2 cell lysates.
    • Western Blotting
      MCA2880GA detects a band of approximately 31kDa in HeLa and HepG2 cell lysates.
    • Instructions For Use
    • Instructions For Use

    Additional NQO1 Antibody Formats

    Formats Clone Applications Sizes available
    NQO1 Antibody : Purified A180 IP, P *, WB 0.1 mg
    NQO1 Antibody : HRP A180 P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human lung
              Human lung
              • Application NameReference Images
                Immunoprecipitation
                Western Blotting

              References

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