NOXO1 Antibody

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Staining of formalin fixed, paraffin embedded human colon with Rabbit anti human NOXO1 (AHP2050)

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  • Rabbit anti Human NOXO1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2050E, IF, P, WBdatasheet pdfdatasheet pdf50 µg
    AHP2050
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human NOXO1 antibody recognizes human NADPH oxidase organizer 1, also known as NOXO1, NADPH oxidase regulatory protein, Nox organizer 1, Nox-organizing protein 1 or SH3 and PX domain-containing protein 5. NOXO1 is a 376 amino acid ~48 kDa cell membrane associated protein involved in the superoxide-generating activity of NOX1 and NOX3 and is required for the development of crystalline gravity sensing structures of the inner ear (Lundberg et al. 2015).

      Multiple isoforms of human NOXO1 generated by alternative splicing have been described. Rabbit anti Human NOXO1 antibody recognizes an epitope within the sequence “SGPQFCASRAYES” representing amino acids 238-252 of isoform 1 of human NOXO1, this sequence is common to all described isoforms of human NOXO1 (UniProt : Q8NFA2).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      ChimpanzeeExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human NOXO1was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.01% Sodium Azide (NaN3)
    • Immunogen
      Recombinant Human NOXO1.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      regulation of respiratory burst
      superoxide-generating NADPH oxidase activator activity
      cell communication
      phosphatidylinositol binding
      regulation of hydrogen peroxide metabolic process
      NADPH oxidase complex
      enzyme binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1:200001:100000
      Immunofluorescence
      Immunohistology - Paraffin510ug/ml
      Western Blotting1:20001:8000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Histology Positive Control Tissue
      Human colon
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NOXO1 Antibody Formats

    Formats Applications Sizes available
    NOXO1 Antibody : Purified E, IF, P, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Histar Detection SystemSTAR3000C500 TestsC, P
          STAR3000C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human colon

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