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NFkB p65 Antibody

NFkB p65 Antibody gallery image 1

Western blot analysis of NFkB IP samples. IP was performed on Jurkat cell lysates using 10 µg (lanes 2 & 7), 5 µg (lanes 3 & 8), 1 µg (lanes 4 & 9) Sheep anti NFkB p65 antibody (AHP394), and 10 µg polyclonal IgG (lanes 5 & 10).

8.75 µl of each IP was loaded onto an AnykD ™ Criterion ™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run lanes 1 & 6.

Mouse anti NFkB p65 (MCA6021) was used at 1/1000 in lanes 1-5. HRP conjugated Goat anti Mouse IgG (H/L) antibody (STAR117P) was used at 1/10,000 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System. Arrow points to NFkB (molecular weight 65 kDa).

  • NFkB p65 Antibody thumbnail image 1
  • Sheep anti Human NFkB p65
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP394E, GS, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Sheep anti Human NFkB p65 antibody recognizes human full length human p65 (NF-kB), also known as Transcription factor p65 or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. Human NFkB p65 is a 551 amino acid plieotropic transcription factor containing a single RHD domain. Sheep anti Human NFkB p65 antibody will also detect proteolytic fragments of NFkB p65 containing the DNA binding RHD domain located in the amino-terminal region. Multiple isoforms of NFkB p65 are generated by alternative splicing. Sheep anti Human NFkB p65 antibody binds to isoforms 1 and 2 containing the intact RHD domain, but it is unclear whether it will bind to isoforms 3 and 4 which have partial differing deletions within the RHD domain.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Gel Super Shift Assays
      Western Blotting1/2001/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NFkB p65 Antibody Formats

    Formats Applications Sizes available
    NFkB p65 Antibody : Purified E, GS, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • NFkB p65 Antibody thumbnail image 1


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