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NFkB p65 Antibody | 2A12A7

NFkB p65 Antibody | 2A12A7 gallery image 1

Western blot analysis of Jurkat cell lysate probed with Mouse anti NFkB (p65) antibody.

NFkB p65 Antibody | 2A12A7 gallery image 2

Western blot analysis of NFkB IP samples. IP was performed on Jurkat cell lysates using 10 µg (lanes 2 & 7), 5 µg (lanes 3 & 8), 1 µg (lanes 4 & 9) Sheep anti NFkB p65 antibody (AHP394), and 10 µg polyclonal IgG (lanes 5 & 10).

8.75 µl of each IP was loaded onto an AnykD ™ Criterion ™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run lanes 1 & 6.

Mouse anti NFkB p65 (MCA6021) was used at 1/1000 in lanes 1-5. HRP conjugated Goat anti Mouse IgG (H/L) antibody (STAR117P) was used at 1/10,000 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System. Arrow points to NFkB (molecular weight 65 kDa).

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  • Mouse anti NFkB p65
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6021IP, P, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti NFkB p65 antibody recognizes Nuclear factor NF-kappa-B p65 subunit also known as Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 or RelA. NF-kappa-B p105 and p100 subunits are large precursor proteins that are cleaved by the ubiquitin/proteasome pathway into the mature NF-kappa-B p50 and p52 subunits (NF-kappa-B p105 cleavage results in the p50 unit, while cleavage of NF-kappa-B p100 results in the p52 subunit). Rel protein family members interact with p52 and p50 to form transcription factors. In the absence of stimulation an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.

      The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
      Western Blotting1.04.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Mouse anti NFkB p65 antibody recognizes a band of approximately 65 kDa in Jurkat cell lysate
    • Instructions For Use

    Additional NFkB p65 Antibody Formats

    Formats Clone Applications Sizes available
    NFkB p65 Antibody : Purified 2A12A7 IP, P, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

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