NFkB p50 Antibody (PrecisionAbTM Antibody)

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NFkB p50 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with NFkB p50 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to NFkB p50 (molecular weight 50 & 120 kDa).

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  • NFkB p50 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti NFkB p50
  • NFkB p50 Antibody with Control Lysate
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    NFkB p50 antibody recognizes nuclear factor NF-kappa-B p50 subunit, also known as DNA binding factor KBF1, NF-kappabeta, nuclear factor NF-kappa-B p50 subunit or nuclear factor kappa-B DNA binding subunit.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00673WBdatasheet pdfdatasheet pdf100 µl
      VMA00673
      VMA00673KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00673KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human NFkB p50 antibody recognizes nuclear factor NF-kappa-B p50 subunit, also known as DNA binding factor KBF1, NF-kappabeta, nuclear factor NF-kappa-B p50 subunit or nuclear factor kappa-B DNA binding subunit.

        NFKB1 encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for NFKB1 (provided by RefSeq, Sep 2009).

        Mouse anti Human NFkB p50 antibody detects bands of 50 and 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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