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NFkB p50 Antibody

NFkB p50 Antibody gallery image 1

Western blot analysis of 3T3 cell lysate (lane 1) and rat kidney tissue lysate (lane 2) probed with Rabbit anti NFkB p50 antibody

  • NFkB p50 Antibody thumbnail image 1
  • Rabbit anti NFkB p50
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2331WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti NFkB p50 antibody recognizes Nuclear factor NF-kappa-B p50 subunit. NF-kappa-B p105 is a large precursor protein that is cleaved at its C-terminus (which mainly contains ankyrin repeats) by the ubiquitin/proteasome pathway into the NF-kappa-B p50 subunit. The NF-kapp- B p50 subunit is also known as NF-kappa-B1 and interacts with members of the Rel protein family to form transcription factors. In the absence of stimulation, an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.
      The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti NFkB (p50) antibody recognizes a band of approximately 50 kDa in 3T3 cell lysate and rat kidney tissue lysate
    • Instructions For Use

    Additional NFkB p50 Antibody Formats

    Formats Applications Sizes available
    NFkB p50 Antibody : Purified WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • NFkB p50 Antibody thumbnail image 1

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