NFIL3 Antibody

NFIL3 Antibody gallery image 1

Western blotting of HepG2 lysate (20µg total protein in RIPA buffer) probed with Goat anti Human NFIL3 antibody (OBT1272) at 0.5μgml-1. Primary incubated for 1 hour. Signal was detected by chemiluminescence

  • NFIL3 Antibody thumbnail image 1
  • Goat anti Human NFIL3
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1272WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human NFIL3 antibody recognizes Nuclear factor interleukin-3-regulated protein (NFIL3), also known as E4 promoter-binding protein 4, Interleukin-3 promoter transcriptional activator, Interleukin-3-binding protein 1 or Transcriptional activator NF-IL3A. NFIL3 is a 462 amino acid ~ 55-60 kDa transcriptional regulator expressed in multiple organs including bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle (Hulme et al. 2000).
    • Intended Use
    • Target Species
    • Product Form
      Ig fraction - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide with the sequence C-RLIATQPISASDSG from the C terminus of the protein sequence according to the Entrez Gene accession number NP_005375
    • Purity
    • Approx. Protein Concentrations
      IgG concentration0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Tris buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      transcription corepressor activity
      sequence-specific DNA binding transcription factor activity
      immune response
      transcription from RNA polymerase II promoter
      regulation of transcription, DNA-dependent
      sequence-specific DNA binding
      rhythmic process
      protein dimerization activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NFIL3 Antibody Formats

    Formats Applications Sizes available
    NFIL3 Antibody : Purified WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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