NFE2L2 Antibody | 3G7

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NFE2L2 Antibody | 3G7 gallery image 1

Western Blot analysis of NFE2L2 expression in PC-12 using Mouse anti Human NFE2L2 (MCA4947Z)

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  • Mouse anti Human NFE2L2:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    3G7
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4947ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA4947Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human NFE2L2 antibody, clone 3G7 recognizes human Nuclear factor erythroid 2-related factor 2, also known as NFE2L2, HEBP1 or nuclear factor erythroid derived 2, like 2. NFE2L2 is a 605 amino acid ~70 kDa transcription activator that binds to antioxidant response elements of target genes. Multiple isoforms of NFE2L2 generated by alternative splicing have been described. All isoforms contain the immunogen sequence used to create clone 3G7. Mouse anti Human NFE2L2 antibody, clone 3G7 is therefore expected to recognize all described isoforms of human NFE2L2 (UniProt : Q16236).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 351-450 of human NFE2L2
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      transcription from RNA polymerase II promoter
      protein dimerization activity
      cytosol
      nucleus
      sequence-specific DNA binding transcription factor activity
      protein domain specific binding
      centrosome
      plasma membrane
      sequence-specific DNA binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NFE2L2 Antibody Formats

    Formats Clone Applications Sizes available
    NFE2L2 Antibody : Preservative Free 3G7 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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