NF Kappa B Inhibitor Alpha Antibody | 6A055

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NF Kappa B Inhibitor Alpha Antibody | 6A055 gallery image 1

Western blot analysis of Jurkat cell lysate treated with (right lane) or without (left lane) TNF alpha (1.0 nM) and probed with Mouse anti NF kappa B inhibitor alpha. The antibody recognizes both the phosphorylated and non-phosphorylated forms of NF kappa B inhibitor alpha

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  • Mouse anti NF Kappa B Inhibitor Alpha
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  • Product Type
    Monoclonal Antibody
  • Clone
    6A055
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6019IP, WBdatasheet pdfdatasheet pdf50 µg
    MCA6019
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti NF kappa B inhibitor alpha antibody, clone 6A055, recognizes NF kappa B inhibitor alpha also known as I-kappa-B-alpha (IkB-alpha) and major histocompatibility complex enhancer-binding protein MAD3. NF kappa B inhibitor alpha is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NF kappa B inhibitor alpha inhibits the NF kappa B complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NF kappa B inhibitor alpha is phosphorylated so that it dissociates from NF kappa B and is then ubiquinated prior to degradation.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation1.04.0 ug/ml
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Mouse anti Human NF Kappa B Inhibitor alpha antibody recognizes both the phosphorylated and non-phosphorylated forms of NF Kappa B Inhibitor alpha (approximately 35 kDa) in untreated Jurkat cell lysate and lysate from Jurkat cells treated with TNFa.
    • Instructions For Use

    Additional NF Kappa B Inhibitor Alpha Antibody Formats

    Formats Clone Applications Sizes available
    NF Kappa B Inhibitor Alpha Antibody : Purified 6A055 IP, WB 50 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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