Neuropeptide Y Receptor 1 Antibody

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Neuropeptide Y Receptor 1 Antibody gallery image 1

Human brain lysate probed with Sheep anti Human Neuropeptide Y Receptor 1 (6732-0150)

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Published customer image:
Sheep anti Human neuropeptide Y receptor 1 antibody used for the detection of NY1R in rat retinal ganglion cells by immunogluorescence.
Image caption:
.NPY and NPY receptor immunoreactivity (ir) in a purified RGC culture obtained from Wistar pups (a–e, red). RGCs were stained with antiBrn3a (RGC marker, green) and nuclei with DAPI (blue). Scale bar: 20?μm.

From: Martins J, Elvas F, Brudzewsky D, Martins T, Kolomiets B, Tralhão P, Gøtzsche CR, Cavadas C, Castelo-Branco M, Woldbye DP, Picaud S, Santiago AR, Ambrósio AF. Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiologyand Exerts Neuroprotective Actions In Vitro.
ASN Neuro. 2015 Aug 26;7(4). pii:1759091415598292.

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Neuropeptide Y Receptor 1 Antibody gallery image 3

Published customer image:
Sheep anti Human neuropeptide Y receptor 1 antibody used for the detection of NY1R in rat retinal ganglion cells by immunogluorescence.
Image caption:
.NPY-ir and NPY receptor-ir in retinal slices obtained from young adult Wistar rats (a–e, red). RGCs were stained with the RGC marker Brn3a (green) and nuclei with DAPI (blue). NPY-ir was mainly detected in strata 1, 3, and 5 of IPL (arrows; a). Y1-ir was detected in distal and proximal INL, strata 2 and 4 of IPL, and RGCs (arrows; b). Y2-ir was detected in INL (c). Y4-ir was detected in INL and GCL (d). and Y5-ir was detected in Müller cells (e). (d) Y5-ir (red) is colocalized with Müller cells (green) in retinal slices of young adult Wistar rats. Müller cells were identified by vimentin-ir (green). Nuclei were stained with DAPI (blue). GCL?=?ganglion cell layer; IPL?=?inner plexiform layer; INL?=?inner nuclear layer; OPL?=?outer plexiform layer; ONL?=?outer nuclear layer. Scale bar: 50?μm.

From: Martins J, Elvas F, Brudzewsky D, Martins T, Kolomiets B, Tralhão P, Gøtzsche CR, Cavadas C, Castelo-Branco M, Woldbye DP, Picaud S, Santiago AR, Ambrósio AF. Activation of Neuropeptide Y Receptors Modulates Retinal Ganglion Cell Physiologyand Exerts Neuroprotective Actions In Vitro.
ASN Neuro. 2015 Aug 26;7(4). pii:1759091415598292.

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  • Sheep anti Human Neuropeptide Y Receptor 1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    6732-0150E, P *, WBdatasheet pdfdatasheet pdf1 ml
    6732-0150
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Human Neuropeptide Y Receptor 1 antibody recognizes human neuropeptide Y receptor 1. Neuropeptide Y receptors are a class of G-protein coupled receptors which are activated by the closely related peptide hormones neuropeptide Y, peptide YY and pancreatic polypeptide. These receptors are involved in control of a diverse set of behavioral processes including appetite, circadian rhythm, and anxiety.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      A synthetic peptide corresponding to amino acids 365 - 378 of human NPY1R, conjugated to KLH.

      Peptide sequence: KQASPVAFKKINNN
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to plasma membrane
      outflow tract morphogenesis
      inhibition of adenylate cyclase activity by G-protein signaling pathway
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1001/1000
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      6732-0150 detects a band of approximately 55 kDa in human brain.
    • Instructions For Use

    Additional Neuropeptide Y Receptor 1 Antibody Formats

    Formats Applications Sizes available
    Neuropeptide Y Receptor 1 Antibody : Purified E, P *, WB 1 ml
    • Copyright © 2016 Bio-Rad

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      STAR88D649GA
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      STAR88F
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      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

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