Purified neuron specific enolase (NSE) has been prepared from human brain tissue by ion exchange chroatography and gel filtration. NSE, also known as gama enolase, 2-phospho-D-glycerate hydro-lyase, enolase-2 or Neural enolase, is a glycolytic enzyme expressed in neuronal tissue and released in response to neural tissue damage. An increase in serum levels of NSE has been found in patients with tumors such as neuroblastoma (Odelstad et al. 1981, Zeltzer et al. 1983).
Purified enzyme from human brain - liquid
Ion exchange chromatography and gel filtration.
See Buffer solution.
50 µmol/min mg at 25ºC determined in 45 mM Imidasole, pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4, 1 mM 2-PG.
SDS PAGE: >95%
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.