Neuron Specific Enolase Antibody | 5G10

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Neuron Specific Enolase Antibody | 5G10 gallery image 1

Human Cortical Neurons stained with Mouse anti Human Neuron Specific Enolase (6720-0827)

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  • Mouse anti Human Neuron Specific Enolase
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  • Product Type
    Monoclonal Antibody
  • Clone
    5G10
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    6720-0827E, P *datasheet pdfdatasheet pdf0.2 mg
    6720-0827
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human neuron specific enolase antibody, clone 5G10 recognizes human neuron specific enolase (NSE), also known as enolase 2, gamma enolase, 2-phospho-D-glycerate hydro-lyase or Neural enolase. NSE is a 433 amino acid ~47 kDa protein, a glycolytic enzyme that is expressed in neuronal tissue and is released as a result of damage to neural tissue. An increase in serum levels of NSE has been found in patients with tumours such as neuroblastoma.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Native neuron specific enolase from brain.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      phosphopyruvate hydratase complex
      glycolysis
      gluconeogenesis
      phosphopyruvate hydratase activity
      magnesium ion binding
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Paraffin(1)1/1001/400
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Brain cortex
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Neuron Specific Enolase Antibody Formats

    Formats Clone Applications Sizes available
    Neuron Specific Enolase Antibody : Purified 5G10 E, P * 0.2 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Brain cortex

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