Mouse anti Human neuron specific enolase (131.1C10) is a monoclonal antibody recognising neuron specific enolase, also known as gamma enolase or enolase 2. Neuron specific enolase is a glycolytic enzyme catalizing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Neuron specific enolase is principally localized to the neuronal cytoplasm (Berger et al. 2002).
Neuron specific enolase is a ~78kDa gamma homodimer, the most acidic isoenzyme of the enolase family. Levels of neuron specific enolase in the cerebrospinal fluid are often elevated in conditions resulting in rapid neuronal destruction such as Creutzfeldt-Jacob Disease (Wakayama et al. 1987).
Tissue Culture Supernatant - liquid
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Instructions For Use
Additional Neuron Specific Enolase Antibody Formats