Nemo-Like Kinase Antibody (PrecisionAbTM Antibody)

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Nemo-Like Kinase Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with nemo-like kinase antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to nemo-like kinase (molecular weight 68 kDa)

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  • Nemo-Like Kinase Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti Nemo-Like Kinase
  • Nemo-Like Kinase Antibody with Control Lysate
  • Nemo-Like Kinase Antibody with Control Lysate
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    Mouse anti human nemo-like kinase antibody recognizes the serine/threonine-protein kinase NLK. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00357WBdatasheet pdfdatasheet pdf100 µl
      VMA00357
      VMA00357KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00357K
      VMA00357KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00357KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human nemo-like kinase antibody recognizes the serine/threonine-protein kinase nemo-like kinase.

        Mouse anti Human nemo-like kinase antibody detects a band of 68 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti nemo-like kinase detects a band of approximately 68 kDa in HEK293 cell lysates
        Anti nemo-like kinase detects a band of approximately 68 kDa in HEK293 cell lysates
        Anti nemo-like kinase detects a band of approximately 68 kDa in HEK293 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Purified His-tagged nemo-like kinase protein
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer
        400μg HEK293 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        Wnt receptor signaling pathway
        ATP binding
        transcription factor binding
        regulation of transcription
        transcription
        cytoplasm
        ubiquitin protein ligase binding
        transforming growth factor beta receptor signaling pathway
        magnesium ion binding
        SH2 domain binding
        nucleus
        serine phosphorylation of STAT3 protein
        negative regulation of Wnt receptor signaling pathway
        MAP kinase activity
        peptidyl-threonine phosphorylation
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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