NEK9 Antibody | 1F6

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NEK9 Antibody | 1F6 gallery image 1

Immunofluorescence showing NEK9 in HeLa cells using Mouse anti Human NEK9:Azide Free (MCA4552Z)

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NEK9 Antibody | 1F6 gallery image 2

Western blot analysis of NEK9 expression in HeLa using Mouse anti Human NEK9:Azide Free (MCA4552Z)

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  • NEK9 Antibody | 1F6 thumbnail image 1
  • NEK9 Antibody | 1F6 thumbnail image 2
  • Mouse anti Human NEK9:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1F6
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4552ZIF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4552Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human NEK9 antibody, clone 1F6 recognizes human Serine/threonine-protein kinase Nek9, also known as NEK9, Nercc1 kinase, Never in mitosis A-related kinase 9, NimA-related protein kinase 9, NimA-related kinase 8 or Nek8. NEK9 is a 978 amino acid ~100 kDa regulator of mitotic progression (Tan and Lee 2003). NEK9 is highly expressed in heart liver kidney and testis. It is also expressed in smooth muscle cells and fibroblasts at the mRNA level.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 226-326 of human NEK9.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NEK9 Antibody Formats

    Formats Clone Applications Sizes available
    NEK9 Antibody : Preservative Free 1F6 IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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