Nck Antibody (PrecisionAbTM Antibody)

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Nck Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with NCK antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 24 second exposure. Arrow points to NCK (molecular weight 47 kDa)

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  • Nck Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti Nck
  • Nck Antibody with Control Lysate
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  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG1
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00589WBdatasheet pdfdatasheet pdf100 µl
    VMA00589
    VMA00589KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00589KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
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    • Mouse anti Human NCK antibody recognizes the cytoplasmic protein NCK1, also known as NCK tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha, melanoma NCK protein or non-catalytic region of tyrosine kinase.

      The protein encoded by NCK1 gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found (provided by RefSeq, Jun 2010).

      Mouse anti Human NCK antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
    • Target Species
      Human
    • Western Blotting
      Anti NCK detects a band of approximately 47 kDa in Jurkat cell lysates
      Anti NCK detects a band of approximately 47 kDa in Jurkat cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
      20μl Mouse monoclonal antibody purified by affinity chromatography Protein G from ascites
    • Buffer Solution
      HEPES buffered saline
      HEPES buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      <50% Glycerol
    • Immunogen
      E. coli-derived recombinant human NCK
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg Jurkat lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      endoplasmic reticulum
      axon guidance
      receptor binding
      T cell receptor signaling pathway
      T cell activation
      signal complex assembly
      cytoskeletal adaptor activity
      cytosol
      positive regulation of actin filament polymerization
      positive regulation of T cell proliferation
      receptor signaling complex scaffold activity
      regulation of translation
      nucleus
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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