Goat anti Human NALP3 antibody recognizes human NACHT, LRR and PYD domains-containing protein 3 (NALP3), also known as Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1 or Cryopyrin. NALP3 is a 1036 amino acid ~120kDa cytoplasmic protein belonging to the NLRP family, containing a single DAPIN domain, nine leucine-rich repeats and a single NACHT domain (UniProt: Q96P20) .
NALP3 is involved in inflammation and immune responses. NALP3 may function as an activator of NF-kappaB signalling and play a role in the regulation of inflammation and apoptosis (Choi and Ryter 2014). NALP3 is strongly expressed in polymorphonuclear cells, osteoblasts and chondrocytes, NALP3 can also be detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
Goat anti Human NALP3 antibody has been used successfuly for the detection of NALP3 in the eyes of rats by immunohistochemistry on formalin fixed, paraffin embedded sections (Liu et al. 2014).
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antisera to human NALP3 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Synthetic peptide sequence corresponding to C-EKPELTVVFEPSW from the C Terminus of Human NALP3 (NP_004886).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.