NAGLU Antibody | 1B7

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NAGLU Antibody | 1B7 gallery image 1

Western blot analysis of NAGLU expression in Jurkat using Mouse anti Human NAGLU:Azide Free (MCA3277Z)

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  • NAGLU Antibody | 1B7 thumbnail image 1
  • Mouse anti Human NAGLU:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1B7
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3277ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA3277Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human NAGLU antibody, clone 1B7 recognizes human Alpha-N-acetylglucosaminidase or N-acetyl-alpha-glucosaminidase. NAGLU is a 720 amino acid ~105 kDa lysosomal glycoprotein involved in the degradation of heparan sulfate.

      Mutations in the NAGLU gene may result in the presentation of the condition Mucopolysaccharidosis 3B (MPS3B) also known as Sanfilippo syndrome type B, a condition characterized by severe central nervous system degeneration (Beesley et al. 1998).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 644-743 of human NAGLU
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      alpha-N-acetylglucosaminidase activity
      nervous system development
      lysosome
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NAGLU Antibody Formats

    Formats Clone Applications Sizes available
    NAGLU Antibody : Preservative Free 1B7 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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