NADPH Oxidase 1 Antibody (PrecisionAbTM Antibody)

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NADPH Oxidase 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with NADPH oxidase 1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 42 second exposure. Arrow points to NADPH oxidase 1 (molecular weight 72 kDa)

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  • NADPH Oxidase 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • NADPH Oxidase 1 Antibody with Control Lysate
  • NADPH Oxidase 1 Antibody with Control Lysate
  • Goat anti NADPH Oxidase 1
(Rated 0.0 out of 5 based on 0 customer reviews)
    NADPH oxidase 1 antibody recognizes NADPH oxidase 1, also known as NOX-1, mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX or NOH-1. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00110WBdatasheet pdfdatasheet pdf100 µl
      VPA00110
      VPA00110KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00110K
      VPA00110KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00110KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human NADPH oxidase 1 antibody recognizes the NADPH oxidase 1, also known as NOX1, mitogenic oxidase 1, MOX1, NADH/NADPH mitogenic oxidase subunit P65-MOX or NOH1.

        Encoded by the NOX1 gene, NADPH oxidase 1 is a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for NOX1 (provided by RefSeq, Nov 2012).

        Goat anti Human NADPH Oxidase 1 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti NOX1 detects a band of approximately 72 kDa in K562 cell lysates.
        Anti NOX1 detects a band of approximately 72 kDa in K562 cell lysates.
        Anti NOX1 detects a band of approximately 72 kDa in K562 cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Goat polyclonal antibody purified by affinity chromatography.
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3) 0.5 % BSA
        0.02% Sodium Azide (NaN3) 0.5 % BSA
        0.02% Sodium Azide (NaN3) 0.5 % BSA
      • Immunogen
        Peptide with the sequence C-DKATDIVTGLKQK, from the internal region of the protein sequence.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer.
        400μg K562 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        angiogenesis
        electron carrier activity
        response to pH
        positive regulation vascular endothelial growth factor production
        positive regulation of integrin biosynthetic process
        cell projection
        early endosome
        hydrogen peroxide metabolic process
        electron transport chain
        signal transduction
        voltage-gated proton channel activity
        superoxide-generating NADPH oxidase activity
        flavin adenine dinucleotide binding
        superoxide anion generation
        iron ion binding
        NADP metabolic process
        Rac GTPase binding
        positive regulation of smooth muscle cell proliferation
        cell migration
        inflammatory response
        NADPH oxidase complex
        respiratory burst
        FADH2 metabolic process
        intracellular pH elevation
        NADP binding
        cell junction
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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