N-Cadherin Antibody (PrecisionAbTM Antibody) | OTI2G7

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N-Cadherin Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI2G7 gallery image 1

Western blot analysis of whole cell lysates probed with N-cadherin antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to N-cadherin (molecular weight 140 kDa)

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  • N-Cadherin Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI2G7 thumbnail image 1
  • Mouse anti N-Cadherin
  • N-Cadherin Antibody with Control Lysate
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    N-Cadherin antibody recognizes N-cadherin-2, also known as N-cadherin 1, cadherin 2, calcium-dependent adhesion protein neuronal or neural cadherin.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00601WBdatasheet pdfdatasheet pdf100 µl
      VMA00601
      VMA00601KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00601KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human N-cadherin antibody recognizes N-cadherin, also known as N-cadherin 1, cadherin 2, calcium-dependent adhesion protein neuronal and neural cadherin.

        N-cadherin is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by CDH2 product (provided by RefSeq, Jul 2008).

        Mouse anti Human N-cadherin antibody detects a band of 140 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti N-cadherin antibody detects a band of approximately 140 kDa in C6 cell lysates
        Mouse anti N-cadherin antibody detects a band of approximately 140 kDa in C6 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
        20μl Mouse monoclonal antibody purified by affinity chromatograpy on Protein A from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        <50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        <50% Glycerol
      • Immunogen
        HEK293-derived full length recombinant human N-cadherin
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg C6 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        adherens junction organization
        muscle cell differentiation
        alpha-catenin binding
        gamma-catenin binding
        calcium ion binding
        beta-catenin binding
        cell junction assembly
        catenin complex
        positive regulation of muscle cell differentiation
        integral to membrane
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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