Myoglobin Antibody | 164.5D5

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Myoglobin Antibody | 164.5D5 gallery image 1

Detection of human myoglobin (PHP156) in sandwich ELISA using Mouse anti Human myoglobin (MCA5879G) as the capture reagent and HRP conjugated Mouse anti Human myoglobin (MCA5874G) as the detection reagent. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®

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  • Mouse anti Human Myoglobin
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  • Product Type
    Monoclonal Antibody
  • Clone
    164.5D5
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5879GEdatasheet pdfdatasheet pdf1 mg
    MCA5879G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human myoglobin, clone 164.5D5 is a monoclonal antibody recognising human myoglobin, a single-chain globular ~17 kDa protein containing a heme prosthetic group localised to skeletal muscle. Myoglobin is responsible for the storage and movement of oxygen in muscle tissue.

      Myoglobin is released from injured muscle into the bloodstream, making it a sensitive marker for all muscle injury. It is commonly used in combination with troponin as a marker for myocardial damage in heart attacks. It is filtered by the kidneys, but is a potential cause of renal failure as it is toxic to the renal tubular epithelium (Lima et al. 2008).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      oxygen transporter activity
      heme binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA5 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Myoglobin Antibody Formats

    Formats Clone Applications Sizes available
    Myoglobin Antibody : Purified 164.5D5 E 1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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