Myocyte-Specific Enhancer Factor 2D Antibody (PrecisionAbTM Antibody) | OTI3D12

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Myocyte-Specific Enhancer Factor 2D Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI3D12 gallery image 1

Western blot analysis of whole cell lysates probed with myocyte-specific enhancer factor 2D antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 15 second exposure. Arrow points to myocyte-specific enhancer factor 2D (molecular weight 70 kDa)

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  • Myocyte-Specific Enhancer Factor 2D Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI3D12 thumbnail image 1
  • Mouse anti Myocyte-Specific Enhancer Factor 2D
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    Myocyte-specific enhancer factor 2D antibody recognizes myocyte-specific enhancer factor 2D, also known as MADS box transcription enhancer factor 2 or polypeptide D. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00268WBdatasheet pdfdatasheet pdf100 µl
      VMA00268
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human myocyte-specific enhancer factor 2D antibody recognizes myocyte-specific enhancer factor 2D, also known as MADS box transcription enhancer factor 2, polypeptide D and myocyte-specific enhancer factor 2D.

        MEF2D encodes a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson's disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants (provided by RefSeq, Oct 2012).

        Mouse anti Human myocyte-specific enhancer factor 2D antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti myocyte-specific enhancer factor 2D detects a band of approximately 70 kDa in RD cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Recombinant protein corresponding to amino acids 1-282 of human myocyte-specific enhancer factor 2D (NP_005911) produced in E. coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        sequence-specific DNA binding transcription factor activity
        nervous system development
        sequence-specific DNA binding
        apoptosis
        nucleus
        protein binding
        muscle organ development
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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