Myelin Proteolipid Protein Antibody | 5691

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Myelin Proteolipid Protein Antibody | 5691 gallery image 1

Trf-PLP probed with Human anti Myelin Proteolipid Protein (PLP1) (HCA094)

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Myelin Proteolipid Protein Antibody | 5691 gallery image 2

Western Blot analysis of CNS tissue using Human anti Human myelin proteolipid protein (HCA094). Image courtesy of Dr. M. Pischetsrieder, University of Erlangen-Nuremberg

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  • Human anti Myelin Proteolipid Protein
  • Human anti Myelin Proteolipid Protein
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5691
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA094E, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA094
    HCA094AE, WBdatasheet pdfdatasheet pdf50 µg
    HCA094A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Myelin Proteolipid Protein antibody, clone 5691 recognizes myelin proteolipid protein (PLP), which is a major constituent protein of central nervous system myelin. The Plp gene is highly conserved across species and encodes two major membrane-bound proteins of the myelin sheath, the large isoform PLP and a smaller isoform DM20. The exact functions of PLP are still unclear but reports suggest that PLP and DM20 may play a role in the formation of the intraperiod line of myelin and in maintaining axonal integrity.

      In man, mutations of the PLP and DM20 proteins have been reported which are associated with X-linked dysmyelinating disorders including the heterogeneous syndromes of Pelizaeus-Merzbacher disease (PMD). The reported mutations vary considerably and range from deletions, missense mutations to additional copies of the gene.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RabbitExpected from Sequence
      DogExpected from Sequence
      BovineExpected from Sequence
      RatExpected from Sequence
      MouseExpected from Sequence
      PigExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      metal chelate affinity chromatography
      metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide corresponding to the sequence CGKGLSATVTGGQKGRGSR of human myelin proteolipid protein.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1001/250
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1001/250

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Myelin Proteolipid Protein Antibody Formats

    Formats Clone Applications Sizes available
    Myelin Proteolipid Protein Antibody : Purified 5691 E, WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
      MCA1396A
      Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
      MCA1396B
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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