MRE11 Antibody (PrecisionAbTM Antibody) | AB02/1D11

//www.bio-rad-antibodies.com/human-mre11-antibody-ab02-1d11-vma00536.html?RenderPageSpecific=1
MRE11 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB02/1D11 gallery image 1

Western blot analysis of whole cell lysates probed with MRE11 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 118 second exposure. Arrow points to MRE11 (molecular weight 81 kDa)

Enlarge
  • MRE11 Antibody (PrecisionAb<sup>TM</sup> Antibody) | AB02/1D11 thumbnail image 1
  • MRE11 Antibody with Control Lysate
  • Mouse anti MRE11
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    PrecisionAb™ Monoclonal
  • Isotype
    IgG1
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VMA00536WBdatasheet pdfdatasheet pdf100 µl
    VMA00536
    VMA00536KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VMA00536KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Mouse anti Human MRE11 antibody recognizes the double-strand break repair protein MRE11A, also known as AT-like disease DNA recombination and repair protein, MRE11 homolog 1, MRE11 homolog A, endo/exonuclease Mre11 or meiotic recombination 11 homolog A.

      MRE11A gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. MRE11A has a pseudogene on chromosome 3. Alternative splicing of MRE11A results in two transcript variants encoding different isoforms (provided by RefSeq, Jul 2008).

      Mouse anti Human MRE11 antibody detects a band of 81 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Anti MRE11 detects a band of approximately 81 kDa in HEK293 cell lysates
      Anti MRE11 detects a band of approximately 81 kDa in HEK293 cell lysates
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      E. coli-derived recombinant human MRE11A (amino acids 182 - 582)
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg HEK293 lysate lyophilized in RIPA buffer
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      double-stranded DNA binding
      DNA duplex unwinding
      regulation of mitotic recombination
      positive regulation of kinase activity
      3'-5' exonuclease activity
      double-strand break repair via nonhomologous end joining
      protein C-terminus binding
      manganese ion binding
      single-stranded DNA specific endodeoxyribonuclease activity
      negative regulation of DNA endoreduplication
      positive regulation of protein autophosphorylation
      sister chromatid cohesion
      Mre11 complex
      reciprocal meiotic recombination
      nucleoplasm
      double-strand break repair via homologous recombination
      telomere maintenance via telomerase
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
      STAR207P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

              Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

              Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

              Contact Information


              Online Payment Methods:

              1. Invoice / Purchase Order
              2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
              credit cards accepted
              • View more products with "MRE11" specificity.